Source of Founding: National Science Centre, PRELUDIUM program, VIII edition
Duration: July 2015 – March 2018
Manager/ Principal Investigator: Olga Urbanek, M.Sc.
Supervisor: Prof. Paweł Łukasz Sajkiewicz, Ph.D.
Participants: Filippo Pierini
Project is mainly focused on influence of electrospinning parameters on chemical composition of nanofibers surface and its effect on efficiency of further surface modification. The main aim of the project is surface modification of the bicomponent nanofibers with chosen polycation. Characteristics of selected properties, both modified and not modified nonwoven, will be conducted. In particular its surface features will be characterized. An additional aim of the study is to determine the effect of surface modified nonwoven on the cell activity, morphology and proliferation in in vitro conditions. Moreover, we will try to determine the optimum composition of nonwovens from the view point of effective surface modification and cell response.
The polymers chosen for fibers formation are commonly used in tissue engineering as scaffolds for skin, nerve or cartilage regeneration. Natural polymer addition increases hydrophilicity and biocompatibility of the material. Also its structure is highly similar to GAG-natural component of cartilage. Synthetic polymer will increase its mechanical properties. Moreover, nanofibers structure is highly similar to ECM components, what additionally may have positive effect on cell response. Biomimetic approach to modify the surface of nanofibers will allow to create complex which naturally occurre in the cartilage. Presence of this complex in the scaffold for cartilage regeneration will allow to control water content in tissue volume, what is the most important for diffusion of nutrients, metabolites and chondrocytes life and activity in cartilage.
Significance of the project/ Motivation
The research planned in this project would contribute the development of science on biomimetic scaffolds made by electrospinning method from polymer blends modified with polycation, naturally occurring in the human tissue. Fundamental research will determine whether the scaffold which was designed is suitable in structure (nanofiber) and chemical composition for the activity and proliferation of chondrocytes. Answers to questions arising from the assumptions of the project at a later term, would contribute to the development of a new medical device used in the cartilage reconstruction.
Articles published in the frame of the project
Urbanek O, Sajkiewicz P, Pierini F. (2017). The effect of polarity in the electrospinning process on PCL/chitosan nanofibres’ structure, properties and efficiency of surface modification. Polymer 124 : 168-175